Derivatized aldehydes were first selectively detected on the basis of their isotopic pattern, then annotated and finally identified by tandem mass spectrometry. Following a metabolomics workflow, the developed methodology was applied to the characterization of aldehyde compounds formed by lipid peroxidation in rats fed two different diets differentially prone to lipoperoxidation. This original approach is based on the introduction of a characteristic isotopic labeling by selective derivatization of the carbonyl function using a brominated reagent. This work aimed at the development of an untargeted approach using high-performance liquid chromatography coupled with high-resolution mass spectrometry (HPLC–HRMS) for tracking aldehydes in both suspect screening and untargeted methods in fecal water, representing the aqueous environment of colon epithelial cells. Lipid peroxidation and subsequent formation of toxic aldehydes, such as 4-hydroxynonenal, is known to be involved in numerous pathophysiological processes, possibly including the development of colorectal cancer. Finally, plasma treated with radical inducer shows elevated aldehyde species compared to untreated plasma. An in vitro model shows how derivatized aldehydes in human and rat plasma are detected.
The system is robust with low intra-/inter-day variation in retention time and peak area. Detection limits of 0.5 nM can be reached for the detection of the derivatized aldehydes. Injection volumes up to 50 microl can be injected without overloading the WCX column.
The WCXE column gives the advantages of direct injection of the sample after protein precipitation and centrifugation into the WCXE-LC-MS/MS system. Using a derivatization cocktail consisting of 4-(2-(trimethylammonio)ethoxy)benzenaminium dibromide (4-APC) and NaBH3CN in the screening and detection of known and unknown aldehyde biomarkers, one can take advantage of the specific fragmentation characteristics of this derivatization reagent in MS/MS. Aldehydes are important as biomarkers for oxidative stress. The system enables the selective purification and re-concentration of the in-vial derivatized aldehydes from plasma and urine samples. If you are a candidate or client looking for more information as to how we can support, please do reach out for a confidential conversation.This paper focuses on the development and optimization of an on-line weak-cation exchange SPE (WCXE) coupled to gradient HPLC with tandem MS detection.
My personal remit is non-sector specific and I have a track record of advising on key appointments ranging from mid to senior level across Scotland. My team and I have experience of recruiting across all roles within the finance and accountancy market, from entry level to c-suite. During my time in recruitment, I’ve partnered with many leading employers with the aim of making the recruitment process as streamlined and enjoyable as possible. I am incredibly passionate about helping candidates secure opportunities in which they will thrive, taking time to understand their career aspirations and goals.
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I am now responsible for leading the specialised Accountancy and Finance division within Professional Services across Edinburgh and Glasgow. I recently joined Search after 5 years working for a global recruitment firm managing their Scottish operations out of Edinburgh.
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